2225 Characterization of D-glucose transport across normal and diabetic retinal vessels

Purpose: Characterization of the D-glucose tnnsport acms isolated normal and diabetic rabtnt retmal vessels wing " in vitro " lriicmpxfusicm techniques. Methods: After manual dissection and caonulation of normal and diabetic retinal vessels, they were penis4 with %D-gluar;e and 'H-inulin and studied under different cqcrimental conditions. Results We observed in normal retinal vessels a significant decrease of the lumen to bath D-ghmse transport by oubain (0. I mWl-p< 0 OS), by phlorizin (0.1 mM-p< 0.01) and in the absence of Na+ m the perfusion and bath solutions (pc 0 01). In diabetic retinal vffsels these inhibitory &cls were not found Conclusions: We showed m B previous work a D-glucose transport acmss rabbit retinal vessels involving a carrier mediated tmntporl system which was increased in diabetic retinal vessels. In this study we demonstrated, for the first time, the existence in normal retinal vessels of a Na+ i~~vohwnznt in this tnmpoa, wluch was not seen in diabetic vessels demonswating an alteration of wxular permeability to glucose in these arterioles xgmentt S96